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Experiment Name:

Chitin treatment of Col wild-type and mutant rre1-1 and mutant rre2-1

Experiment Description:

Arabidopsis thaliana seeds were surface sterilized. Approximately 500 seeds(10 microgram) were placed in 250 mL erlenmeyer flasks containing 125 mL of liquid Murashige and Skoog medium (Sigma, St Louis, MO, USA) containing 2% dextrose. These flasks were incubated with gentle shaking for 14 days under constant illumination (125 ”Einsteins m-2 s-1) at 22șC. After this time, acid-hydrolysed, crab-shell chitin (Sigma, St Louis, MO, USA) was added to give a final concentration 100 mg/L. The control flasks received an equivalent amount of sterile, double-distilled water for each treatment. Flasks were incubated for 30 minutes with the crab-shell chitin. Whole seedlings from each treatment were collected, the medium was drained, and the seedlings were rapidly forzen in liquid nitrogen. The results of four replicates are presented.

Starting Date (mm/dd/yyyy):

12/01/2004

Researcher:

Bi-Huei Hou

Genomics/Proteomics Site:

Stanford University

Genomics/Proteomics Investigator:

Shauna Somerville

Experiment Type:

transcriptional profiling
Array Name Array Type Sample Used To Make This Extract Sample Description Source For This Sample (Target) Treatment Used To Make This Extract Treatment Category Treatment Time (Hours) Files Available For Download For This Array
Col_C1 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana untreated elicitor 0.5

Col_C1.DAT

Col_C1.CEL

Col_C1.EXP
Col_C2 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana untreated elicitor 0.5

Col_C2.CEL

Col_C2.EXP

Col_C2.DAT
Col_C3 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana untreated elicitor 0.5

Col_C3.DAT

Col_C3.EXP

Col_C3.CEL
Col_C4 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana untreated elicitor 0.5

Col_C4.EXP

Col_C4.CEL

Col_C4.DAT
Col_Chitin1 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana chitin treated elicitor 0.5

Col_Chitin1.DAT

Col_Chitin1.CEL

Col_Chitin1.EXP
Col_Chitin2 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana chitin treated elicitor 0.5

Col_Chitin2.EXP

Col_Chitin2.DAT

Col_Chitin2.CEL
Col_Chitin3 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana chitin treated elicitor 0.5

Col_Chitin3.CEL

Col_Chitin3.DAT

Col_Chitin3.EXP
Col_Chitin4 Affymetrix Col-0 Genotype: Columbia-0, wild-type Arabidopsis thaliana chitin treated elicitor 0.5

Col_Chitin4.EXP

Col_Chitin4.DAT

Col_Chitin4.CEL
RRE1_C1 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE1_C1.CEL

RRE1_C1.EXP

RRE1_C1.DAT
RRE1_C2 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE1_C2.EXP

RRE1_C2.CEL

RRE1_C2.DAT
RRE1_C3 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE1_C3.DAT

RRE1_C3.EXP

RRE1_C3.CEL
RRE1_C4 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE1_C4.CEL

RRE1_C4.EXP

RRE1_C4.DAT
RRE1_Chitin1 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE1_Chitin1.CEL

RRE1_Chitin1.EXP

RRE1_Chitin1.DAT
RRE1_Chitin2 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE1_Chitin2.DAT

RRE1_Chitin2.CEL

RRE1_Chitin2.EXP
RRE1_Chitin3 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE1_Chitin3.DAT

RRE1_Chitin3.EXP

RRE1_Chitin3.CEL
RRE1_Chitin4 Affymetrix RRE1 Genotype: rre1-1 (rapid response to elicitor 1-1), a T-DNA insertion mutant (SALK_066755). RRE1 (At2g35000) enocdes a protein with a RING domain found in ubiqitin E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE1_Chitin4.DAT

RRE1_Chitin4.EXP

RRE1_Chitin4.CEL
RRE2_C1 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE2_C1.EXP

RRE2_C1.CEL

RRE2_C1.DAT
RRE2_C2 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE2_C2.CEL

RRE2_C2.DAT

RRE2_C2.EXP
RRE2_C3 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE2_C3.EXP

RRE2_C3.CEL

RRE2_C3.DAT
RRE2_C4 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana untreated elicitor 0.5

RRE2_C4.CEL

RRE2_C4.EXP

RRE2_C4.DAT
RRE2_Chitin1 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE2_Chitin1.EXP

RRE2_Chitin1.CEL

RRE2_Chitin1.DAT
RRE_Chitin2 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5 There is no file for this array yet.
RRE2_Chitin2 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE2_Chitin2.CEL

RRE2_Chitin2.EXP

RRE2_Chitin2.DAT
RRE2_Chitin3 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE2_Chitin3.DAT

RRE2_Chitin3.CEL

RRE2_Chitin3.EXP
RRE2_Chitin4 Affymetrix RRE2 Genotype: rre2-1 (rapid response to elicitor 2-1), a T-DNA insertion mutant (SALK_133789). RRE2 (At3g16720, alias = ATL2) encodes a protein with a RING domain found in E3 ligases. Arabidopsis thaliana chitin treated elicitor 0.5

RRE2_Chitin4.EXP

RRE2_Chitin4.CEL

RRE2_Chitin4.DAT

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