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Experiment Name:

Treatment of Columbia wild-type seedlings with crab-shell chitin and chitin octamer

Experiment Description:

Arabidopsis seeds (~30 seeds per 10 ml culture) were surface sterilized and allocated in 50 ml Falcon tubes containing 10 ml of liquid medium (1 X Murashige and Skoog Basal Salt Mixture + 2% Dextrose, pH 5.8). The tubes were incubated with gentle shaking in a growth chamber with the following settings: constant light at 125 µmol m-2 sec-1 at a temperature of 23 °C. Gentle shaking was employed to minimize the possible induction of genes by touch or physical injury. After 14 days, chitin was added to the medium containing the seedlings at the following concentrations: hydrolyzed crab-shell chitin (Sigma-Aldrich, St. Louis, Missouri) at a final concentration of 100 µg/ml or the purified chito-octamer (d.p. =8) at a final concentration of 1 µM. The control seedlings were similarly treated with an equivalent amount of H2O. Whole seedlings were collected 30 minutes after treatment, immediately frozen in liquid N2, and stored at –80 °C for later RNA isolation. Three independent replicates were conducted.

Starting Date (mm/dd/yyyy):

05/10/2005

Researcher:

Katrina Ramonell

Genomics/Proteomics Site:

Stanford University

Genomics/Proteomics Investigator:

Shauna Somerville

Experiment Type:

transcriptional profiling
Array Name Array Type Sample Used To Make This Extract Sample Description Source For This Sample (Target) Treatment Used To Make This Extract Treatment Category Treatment Time (Hours) Files Available For Download For This Array
Crab-shell chitin 1 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Crab-shell chitin elicitor 0.5

Chitin mix_1.EXP

Chitin mix_1.CEL
Crab-shell chitin 2 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Crab-shell chitin elicitor 0.5

Chitin mix_2.CEL

Chitin mix_2.EXP
Crab-shell chitin 3 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Crab-shell chitin elicitor 0.5

Chitin mix_3.CEL

Chitin mix_3.EXP
Chitin octamer 1 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Chitin Octamer (8mer) elicitor 0.5

8mer_1.EXP

8mer_1.CEL
Chitin octamer 2 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Chitin Octamer (8mer) elicitor 0.5

8mer_2.CEL

8mer_2.EXP
Chitin octamer 3 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Chitin Octamer (8mer) elicitor 0.5

8mer_3.CEL

8mer_3.EXP
Water control 1 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Water control elicitor 0.5

Mock_1.CEL

Mock_1.EXP
Water control 2 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Water control elicitor 0.5

Mock_2.CEL

Mock_2.EXP
Water control 3 Affymetrix Col-0 Columbia wild-type seedlings grown hydroponically; 14 days old. Arabidopsis thaliana Water control elicitor 0.5

Mock_3.CEL

Mock_3.EXP

Description     Treatment List     Sample List     Array List (Raw Data)    File List (Normalized Data And Other Information)
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